Ctcf zinc finger folding cristallography

WebJun 28, 2024 · CTCF is a multivalent 11-zinc finger DNA-binding protein that is ubiquitously expressed in most tissues of vertebrate species . Combinatorial usage of CTCF zinc fingers (4, 5) allows its binding to tens of thousands of conserved DNA sites as revealed by chromatin immunoprecipitation sequencing (ChIP-seq) experiments . More than 30% and … WebFeb 16, 2024 · Silencing CTCF or COL1A1/COL1A3 markedly inhibited cell growth and migration in the metastatic GC cell line BGC823. Collectively, this ECM-related 7-gene signature provides a novel insight for survival prediction among GC patients. The zinc finger protein CTCF regulates ECM-related genes, thereby promoting GC cell growth …

CTCF participates in DNA damage response via poly(ADP …

WebJun 5, 2015 · CCCTC-binding factor (CTCF) is a highly conserved zinc finger protein and is best known as a transcription factor. It can function as a transcriptional activator, a repressor or an insulator ... lithium ion forklift https://portableenligne.com

Dynamic Nature of CTCF Tandem 11 Zinc Fingers in Multivalent ...

WebHowever, the 3′ end of the CTCF motif is occupied by the N-terminal CTCF zinc fingers , and cohesin appears to bind the CTCF C terminus . Finally, CTCF may form local secondary DNA structures ( 83 ), which have been invoked to account for the directionality of loop formation between CTCF binding sites ( 102 ). WebAug 31, 2024 · CCCTC-binding factor (CTCF) is a zinc-finger protein, serving an important part in the genome architecture as well as some biochemical processes. Over 70,000 … WebThe endogenous CTCF migrates as a 130 kDa (CTCF-130) protein on SDS-PAGE, however, the open reading frame (ORF) of the CTCF cDNA encodes only a 82 kDa … lithium ion form

CTCF and CTCFL in cancer - ScienceDirect

Category:CTCF - an overview ScienceDirect Topics

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Ctcf zinc finger folding cristallography

CTCF and Cohesin in Genome Folding and Transcriptional Gene …

WebJul 2, 2024 · The 11 zinc fingers (ZFs) of the transcription factor CTCF play a versatile role in the regulation of gene expression. CTCF binds to numerous genomic sites to form … WebThe 11 zinc fingers (ZFs) of the transcription factor CTCF play a versatile role in the regulation of gene expression. CTCF binds to numerous genomic sites to form chromatin …

Ctcf zinc finger folding cristallography

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WebDNA binding by CTCF zinc fingers in human genome architecture ... genes for investigating 3D genome folding and gene reg-ulation (Supplementary information, Figure S1A) [3, 20]. WebCTCF is an evolutionarily conserved zinc finger (ZF) phosphoprotein that binds through combinatorial use of its 11 ZFs to ∼50 bp target sites that have remarkable sequence variation. Formation of different CTCF–DNA complexes, some of which are methylation-sensitive, results in distinct functions, including gene activation, repression, silencing and …

WebCTCF is also a tumour suppressor frequently mutated in cancer, however, the structural and functional impact of mutations have not been examined. We performed molecular and … WebThe CTCF zinc finger domain is required for recruitment to DSB Numerousproteinsinvolved inthe DNA repairprocessare PARylated (23), and this posttranslational modifi cation can play a significant role in the recruitment of proteins to DNA breakages ( 9, 29). We observe that the association of CTCF with PARylation is substantially enriched

WebFeb 16, 2024 · The zinc finger protein CTCF regulates ECM-related genes, thereby promoting GC cell growth and migration. Gastric cancer (GC), a leading cause of cancer-related death, is a heterogeneous disease. ... WebThe sites 45 to 47 correspond to CTCF zinc fingers 1 to 3. Based on the analysis combined with Figure 4, there were significant sequence differences observed between the positive …

WebJun 5, 2015 · CCCTC-binding factor (CTCF) is an 82-kDa protein with 11 zinc fingers. 1 It was first identified as a transcriptional repressor of the chicken c-myc gene, a regulatory gene that encodes the c-myc ...

WebMar 6, 2024 · However, the role of CTCF in DNA damage response remains elusive. Here, we show that CTCF is quickly recruited to the sites of DNA damage. The fast recruitment is mediated by the zinc finger domain and poly (ADP-ribose) (PAR). Further analyses show that only three zinc finger motifs are essential for PAR recognition. lithium ion formationWebDifferent subsets or combination of individual zinc fingers gives the ability to CTCF to recognize multiple DNA target sites. Sequence similarities. Belongs to the CTCF zinc-finger protein family. Curated. Keywords. Domain. #Repeat; #Zinc-finger; Phylogenomic databases. GeneTree. ENSGT00940000156672; HOGENOM. CLU_002678_77_1_1; … impurity\\u0027s mfWebMar 1, 2012 · The linker domains of the CTCF zinc finger domain were found to be phosphorylated during mitosis. The phosphorylation of linker domains impaired the … impurity\\u0027s mjTranscriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. CTCF is involved in many cellular processes, including transcriptional regulation, insulator activity, V(D)J recombination and regulation of … See more CCCTC-Binding factor or CTCF was initially discovered as a negative regulator of the chicken c-myc gene. This protein was found to be binding to three regularly spaced repeats of the core sequence CCCTC and thus … See more The primary role of CTCF is thought to be in regulating the 3D structure of chromatin. CTCF binds together strands of DNA, thus forming chromatin loops, and anchors DNA to cellular structures like the nuclear lamina. It also defines the boundaries between active and … See more CTCF binds to itself to form homodimers. CTCF has also been shown to interact with Y box binding protein 1. CTCF also co-localizes with cohesin, which extrudes chromatin loops by actively translocating one or two DNA strands through its ring-shaped structure, … See more • CCCTC-binding+factor at the U.S. National Library of Medicine Medical Subject Headings (MeSH) • FactorBook CTCF See more The binding of CTCF has been shown to have many effects, which are enumerated below. In each case, it is unknown if CTCF directly evokes the outcome or if it does so indirectly (in … See more CTCF binds to the consensus sequence CCGCGNGGNGGCAG (in IUPAC notation). This sequence is defined by 11 zinc finger motifs in its structure. CTCF's binding is disrupted by CpG methylation of the DNA it binds to. On the other hand, CTCF binding may … See more • Ohlsson R, Renkawitz R, Lobanenkov V (2001). "CTCF is a uniquely versatile transcription regulator linked to epigenetics and disease". Trends Genet. 17 (9): 520–7. doi:10.1016/S0168-9525(01)02366-6. PMID 11525835. • Klenova EM, Morse … See more impurity\u0027s mkWebJul 29, 2024 · The 11 zinc finger (ZF) protein CTCF regulates topologically associating domain formation and transcription through selective binding … impurity\u0027s miWebDec 31, 2016 · The linker domains of the CTCF zinc finger domain were found to be phosphorylated during mitosis. The phosphorylation of linker domains impaired the DNA-binding activity in vitro. Mutation analyses showed that amino acid residues (Thr289, Thr317, Thr346, Thr374, Ser402, Ser461, and Thr518) located in the linker domains … lithium ion forklift battery manufacturersWebTranscription from the amyloid precursor protein (APP) promoter is largely dependent on a nuclear factor binding site designated as APBβ. The protein that binds to this site is the … lithium ion forklift battery sds