WitrynaIn the last decade, the number of pesticides used to ensure the safety of our food and water has grown significantly, and the nature of pesticides and food matrices are becoming increasingly more complex. WitrynaThe hybrid LBA/LC-MS is a semiquantitative assay with acceptable specificity, drug tolerance and the capability of multiplexed detection of ADA isotypes. The assay …

Understanding the role of immunoaffinity-based mass …

WitrynaAbstract: This study established an immunoaffinity column for selective extraction of aflatoxins in agri-products. Specifically, the immunoaffinity column was developed by covalently coupling monoclonal antibody 1C11 against aflatoxins to amino-silica gel microparticles and then packing these into a cartridge. The extraction conditions were ipmc study guide

Quantitation of Insulin Glargine and Major Metabolites

Witryna(LC-MS/MS). In this work, both 1,25D 2 and 1,25D 3 were extracted from human plasma using immunoaffinity extraction and quantified with LC-MS/MS. Goal To validate a very sensitive LC-MS/MS method to quantify 1,25-dihydroxyvitamin D by combining immunoaffinity extraction and highly selective atmospheric pressure chemical … WitrynaExpertise in the use of LC-MS for small molecule or large molecule bioanalysis and biotransformations and the application of hybrid … WitrynaAutomating a hybrid LC-MS/MS sample preparation approach for highly sensitive and reproducible protein quantification. ... Immunoaffinity reagents: Magne Protein A Beads (Promega, G8781) were used for the purification of infliximab. Tris Buffered Saline (TBS) (25 mM Tris, 150 mM NaCl, pH 7.2) was used as the wash/bind buffer and was … ipmc sophia